Evaluation of two diluents of refrigeration and the effect of the presence of the seminal plasma on the viability of cooled equine semen

Authors

  • Rossane Pereira da Silva
  • Karen Martins Leão
  • Elen de Paula Matias Moutinho
  • Natalia do Carmo Silva
  • Moraima Castro Rodrigues
  • Marco Antônio Pereira da Silva

DOI:

https://doi.org/10.22256/pubvet.v5n20.1126

Keywords:

horse, reproduction, artificial insemination, spermatic viability

Abstract

The objective of the work was evaluate the effectiveness of two commercial diluents of refrigeration, Botu-SemenÒ and EquimixÒ, and the effect of the presence of the seminal plasma on the viability of the cooled equine semen. Was used twenty five ejaculates for refrigeration in six protocols. Integrity of plasmatic membrane (%) was evaluated after 24 (T24) hours of refrigeration and total motility (%), progressive motility (%) and vigor (0 the 5) soon after the preparation of samples (T0) and after 24 (T24), 48 (T48), 72 (T72) hours of refrigeration. The semen samples were stored in refrigerator 5ºC. Concluded that not have effect of the diluents tested and the presence of the seminal plasma on the parameters evaluated of the cooled equine semen up to 72 hours of refrigeration 5°C.

Published

2015-09-13

Issue

Section

Reprodução animal

How to Cite

Evaluation of two diluents of refrigeration and the effect of the presence of the seminal plasma on the viability of cooled equine semen. (2015). Pubvet, 5(20). https://doi.org/10.22256/pubvet.v5n20.1126

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