Comparative technique of ovarian biopsy in mares

Abstract

The cyclic changes in reproduction and behavior of mares may be related, in considerable numbers, ovarian changes. Comparative procedures with the standard form of blood sampling for biopsy, when surgical excision or necropsy, determine quality in evaluating the results. The sample collection for ovarian biopsy using a needle sharp Trucut type, was tested in randomly selected pieces of slaughterhouse Itapetinga in Itapetinga - BA. Defining tissue planes by the morphology of the provision (cellular dense, turgid and collagenized), and performing the comparison of results in relation to the standard form of harvest, which did not differ significantly, demonstrating the representativeness of the new technique. Three modes of assessment were used to compare the tissue planes within the same sample (percentage of area occupied by cells, the organizational level of cells, chromaticism of the cell nucleus), resulting in significant differences between the areas of tissue planes per sample. We conclude that this is a new approach to the use of less invasive procedures and trauma, when the sample collection for ovarian biopsy in mares and may eventually join the minimally invasive surgeries, such as for transluminal endoscopic surgery natural orifices (NOTES).