Comparative analysis of DNA extraction protocols in free-range chickens with respect to efficiency, extraction facility and cost
DOI:
https://doi.org/10.22256/pubvet.v9n11.483-489Keywords:
birds, costs, DNA, PCR, sexing.Abstract
The objective of this paper was to test three different protocols for DNA extraction regarding the extraction efficiency, quantity, quality of DNA extracted and extraction cost. Animals were from a farm in Rio de Janeiro state. Feathers samples of free-range chickens were collected from pectoral and blood samples were collected from brachial vein. The protocols tested were: 1) Rapid alkaline extraction from total blood collect; 2) Rapid alkaline extraction from feathers bulb of pectoral; and 3) DNA extraction with blood commercial kit (Nucleo Spin® Tissue) in FTA Card (NucleoCard). After the extraction, samples were quantified e the concentration and quality of DNA were verified in all protocols, using spectrophotometry. The protocols 1 and 2 had good result in the amount of DNA extracted, but they had high concentration of proteins. Protocol 3 had low amount of DNA extracted, but it has high purity. All protocols had good results in relation to the quality of amplification, although, protocols 1 and 2 of alkaline extraction had more facility to use, higher speed in extraction and lower cost than protocol 3.
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Copyright (c) 2015 Aylton Bartholazzi Junior, Djalma Sthel Torres, Celia Raquel Quirino, Thiago Corrêa Silva, Aline Pacheco, Steveen Ribeiro Leal, Dayana Rangel Falcão, Aparecida De Fátima Madella-Oliveira
This work is licensed under a Creative Commons Attribution 4.0 International License.
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