Antitumor activity of Hymenaea martiana hayne leaf in mammary cells of dogs

Abstract

Neoplasms of the mammary gland in dogs are a major cause of death in canines due to treatment limitations and the complexity of surgical procedures. Therefore, new alternatives are needed, such as the crude ethanolic extract from the H. martiana leaf, which has antimicrobial, anti-inflammatory and cicatrizant activity. However, little is known about its antitumor effects and primarily on canine tumor cells. The objective of the study was to evaluate the antitumor and cytopathic effect of the crude ethanolic extract of the H. martiana Hayne leaf on dog tumor cells and VERO cells. In this study, we obtained an action of the extract in front of the tumor cells as the concentration of the extract increased. In addition, macroscopic alterations were seen, such as vacuoles formation, nucleus condensation and cell formation. When the MTT test was performed, mitochondrial function decreased as the phytotherapeutic concentration increased (P<0.05). Thus, H. martiana Hayne can be thought of as an alternative because its bioactive components have been shown to be efficient against tumor cells.