Motility of bovine semen incubated in a water bath or thermos

Abstract

We evaluated two doses of bovine semen, in domestic and imported, incubated by two methods, with the aim of quantifying the motility of sperm. After thawing, the semen was incubated in a water bath and the flask at a temperature of 37 º C for 60 minutes, and the evaluations of sperm motility performed every 20 minutes. The results of motility of sperm were incubated in a water bath, respectively 50, 30, 20 and 20%, 40, 30, 10, and 10% for domestic and imported semen after 0, 20, 40 and 60 minutes. The semen incubated in thermos results showed sperm motility 40, 20, 10 and 10% and 40, 10, 10 and 10% for domestic and imported semen in their times of 0, 20, 40 and 60 minutes after thawing. There was no statistical difference (chi-square) significant (p> 0.05) between semen incubated in thermos or water bath, or between the origin of the semen (nationality or imported). We conclude that while spending time sperm motility decreases, even though the water temperature constant, while maintaining sperm motility after 60 minutes of thawing.