Effect of prostaglandin E2 dose on ovulation in prepubertal mice: a pilot study

Abstract

The objective of this study was to evaluate the effect of different doses of prostaglandin E2 (PGE2) on the ovulation of prepubertal female mice. Eighteen In BALB/c prepubertal mice were treated with 5 IU of intraperitoneal (i.p.) eCG on day 0. On day 2, the mice were randomly distributed into 6 groups for the i.p. administration of: 1) 0.5 mL of PBS (n = 3), 2) 5 µg of GnRH (n = 3), 3) 25 µg of PGE2 (n = 3), 4) 50 µg of PGE2 (n = 3), 5) 150 µg of PGE2 (n = 3) e 6) 250 µg of PGE2 (n=3). On day 3, the mice were killed and the oviducts were collected for the oocyte count by transillumination technique under stereomicroscopy. There were no difference in the number of ovulated mice and number of ovulated oocyte in the females treated with GnRH and 250 µg de PGE2, and both groups showed differences in comparison with PBS group (P<0.001). The results demonstrated that the PGE2 dose of 250 µg induces the ovulation in prepubertal female mice.