Evaluation of benzazolic fluorescent dye for the detection of parasites diagnosed by simple flotation

Authors

  • Sandra Marques Universidade Federal do Rio Grande do Sul
  • Hélio Lopes Barbosa Barros Universidade Federal do Rio Grande do Sul,
  • Raquel Glasenapp Universidade Federal do Rio Grande do Sul,
  • Valter Stefani Universidade Federal do Rio Grande do Sul,

DOI:

https://doi.org/10.22256/pubvet.v12n4a73.1-5

Keywords:

Benzazole, biomarkers, fluorescent dye, parasites

Abstract

This study shows the use of 4-hydroxy-3- (6-methylbenzo [d] oxazol-2-yl) benzenesulfonic acid (HB-9), a fluorescent dye, for the detection and visualization of helminth eggs and protozoan oocysts diagnosed in domestic animals, by epifluorescence microscopy. Eggs of Acuaria spiralis, Strongyloides papillosus, Capillaria spp., Bertiella spp., Strongyloidea and Eimeria spp., were previously identified through the flotation method and used for analysis. The fluorescence of the eggs was visualized in a BEL R Photonics com sistema de Epi-FluorescênciaINV-100 FL Plus. Only eggs of Acuaria spiralis,  Capillaria spp., and Bertiella spp. presented fluorescence when visualized by epifluorescence microscopy. The results indicate that the fluorescente dye used in this study permits a easy, clear and selective detection and visualization ofparasitic structures.

Published

2018-04-17

Issue

Section

Medicina veterinária

How to Cite

Evaluation of benzazolic fluorescent dye for the detection of parasites diagnosed by simple flotation. (2018). Pubvet, 12(04). https://doi.org/10.22256/pubvet.v12n4a73.1-5

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