Vibility of isolates of Malassezia pachydermatis and Rhodotorula spp. in distilled water

Abstract

Biological collections has a propose to store various ecosystem organisms in laboratories for identification and research. For fungi it is necessary to be careful about which methods of conservation to use, because they have different genres and peculiarities. Among the methods of storage, Castellani (distilled water) is considered a simple form and get good results for its use in the storage of microorganisms in vitro. Therefore, it was proposed the study with the purpose of evaluating the viability of Malassezia pachydermatis and Rhodotorula spp. stored by the Castellani method. Four isolates of M. pachydermatis and three isolates of Rhodotorula spp. by the Center of Diagnosis and Research in Veterinary Medicine (MicVet). The isolates were stored in test tubes containing sterile distilled water for six months at room temperature. Subsequently the tubes were lightly stirred and an aliquot of the solution was seeded in Petri dish containing ideal culture for each fungal species. In the working conditions, all isolates of Rhodotorula spp. were recovered, whereas no isolates of M. pachydermatis had growth. In conclusion, the method of conservation in distilled water was viable for Rhodotorula spp., but it is not suitable for M. pachydermatis.