Bladder microbiota of primiparous sows for slaughter

Abstract

Urine samples from 190 primiparous sows from farms located in the states of Parana, Mato Grosso do Sul and Santa Catarina, sent to a slaughterhouse located in the northwestern region of Paraná State were examined. During nine months, samples of 5 mL of urine were randomly collected, obtained directly from the bladder, with a sterile syringe, put in a test tube and transported to the laboratory in isothermal boxes. The samples were incubated within agar base plus 5 to 8% of defibrinated sheep blood and MacConkey agar and then incubated at 37°C for 24-36h, in order to isolate pathogens. The samples that growth were subjected to macroscopic analysis of colonies. Moreover, the microscopic characteristics were observed through the Gram stain and biochemical tests. Presence of microorganisms was found in 147 (77.36%) samples. Bacterial agents, isolated in cultures of studied urine samples, were Escherichia coli 102 (53.68%), Enterobactericiae 30 (15.78%), Hafnia alvei 7 (3.68%), Streptococcus sp. 3 (1.57%), Kluyera species 2 (1.05%), Edwardsiella tarda 1 (0.52%), Erwinia herbicola 1 (0.52%), Staphylococcus sp.1 (0.52%). The aim of this study was to characterize the bladders microbiota of primiparous sows for slaughter.