Occurrence of field fungi and storage and feed ingredients for tambaqui (Colossoma macropomum)

Abstract

The control of microbial growth in feed used in animal feed is a way of maintaining quality hygienic and thereby prevent the emergence of undesirable changes. The present work aims to verify the occurrence of field fungi and storage in a fish and feed for their constituents, and to detect the level of contamination by field fungi and storage: samples of corn bran and soybean, mesquite flour, vitamin and mineral supplement (PREMIX) used in formulating a ration for fish, and in the same samples, stored, 0, 30.60 and 90 days. The experiments were conducted at the Centro de Ciências Agrárias, Univesidade Federal de Alagoas, in the laboratory of Agricultural Microbiology. Ration and its constituents were supplied by the Laboratory of Nutrition of Aquatic Organisms. For isolation of fungi was used the method of serial dilutions followed by seeding in Petri dishes containing culture medium and incubated in darkness at 28°C, when calculated the number of Colony Forming Units (CFU). Among the constituents the greatest number of CFU was detected in Premix and mesquite flour (respectively 4.41x10⁴ and 4.36x10⁴) did not differ statistically among themselves. Regarding % mesquite added to the diet, the highest number of CFU was found among the patients treated 0% and the lowest with 50%. With respect to storage time the greatest number of CFU at time 0 was the lowest at 30 days. Considering the interaction between the two factors it appears that the greatest number of CFU occurred at time 0 for treatment without mesquite flour (7.37 x10⁴) while the lowest was detected at 30 days of storage for 50 and 100% added mesquite (1.52 x10⁴). We identified the field fungi Bipolaris and Cladosporium, Aspergillus and Penicillium and storage.