Evaluation of the Ki-67, p53 in malignant mammary tumors in the bitch
DOI:
https://doi.org/10.22256/pubvet.v6n16.1361Keywords:
neoplasia; mammary tumors; dogs; markers; prognostic.Abstract
In view of the high incidence of mammary neoplasia in dogs, the aim of this study was to investigate the prognostic markers in the malignant mammary tumors of the female dog. For this, a sample of 30 mammary tumors was selected. The samples were divided into three groups according to histopathological classification: carcinosarcoma, solid carcinoma or tubulopapillary carcinoma. The Ki-67, p53, prognostic markers were evaluated by means of immunohistochemistry, using the streptavidin-biotin-peroxidase technique. For Ki-67, a significant difference was seen between solid and tubulopapillary carcinomas. The p53, a significant difference was seen between carcinosarcoma and solid anda tubulopapillary carcinomas.
References
BENJAMIN, S. A.; LEE, A. C.; SAUNDERS, W. J. Classification and behavior of canine mammary epithelial neoplasms based on life-span observations in beagles. Veterinary Pathology, v. 36, p. 423-436, 1999.
COTRAN, R. S.; KUMAR, V.; COLLINS, T. Robbins Patologia Estrutural e Funcional. 6. ed. Rio de Janeiro: Guanabara Koogan, 2000. p. 979-1002.
GASPARINI, G.; BORACCHI, P.; VERDEIRO, P.; BEVILACQUA, P. Cell kinetics in human breast câncer: comparison between the prognostic value of the cytofluorimetric S-phase fraction and that of the antibodies Ki-67 and PCNA antigens detected by immunocytochemistry. International Journal of Cancer, v. 57, p. 822-829, 1994.
GERDES, J.; ZENTGRAF, H. Ki67/MIB1 immunostaining in tumor pathology. European Journal of Histology, v. 42, p.15, 1998.
LEE, C. H.; KIM, W. H.; LIM, J. H.; KANG, M. S.; KIM, D. Y.; KWEON, O. K. Mutation and overexpression of p53 as a prognostic factor in canine mammary tumors. Journal of Veterinary Science, v. 5, n. 1, p.63-69, 2004.
LOWE, S. W.; BODIS, S.; MACCLATCHEY, A. P53 status and the efficacy of cancer therapy in vivo. Science, n. 266, p. 807-810, 1994.
MARTÍN de LAS MULAS, J.; MILLÁN, Y.; DIOS, R. A prospective analysis of immunohistochemically determined estrogen receptor and progesterone receptor expression and host ant tumor factors as predictors of disease-free period in mammary gland of the dog. Veterinary Pathology, v.42, p. 200-212, 2005.
MAY, P.; MAY, E. Twenty years of p53 research: structural and functional aspects of the p53 protein. Oncogene, v. 8, p. 7621-7636, 1999.
MAYR, B.; DRESSLER, A.; REIFINGER, M.; FEIL, C. Cytogenetic alterations in eight mammary tumors and tumor-supressor gene p-53 mutation in one mammary tumor from dogs. American Journal of Veterinary Research, v.59, p. 69-78, 1998.
MILLANTA, F.; CALANDRELLA, M.; BARI, G.; NICCOLINI, M.; VANNOZZI, I.; POLI, A. Comparison of steroid receptor expression in normal, dysplastic, and neoplastic canine and feline mammary tissues. Research in Veterinary Science, v.79, p. 225-232, 2005.
MILLANTA, F.; CITI, S.; DELLA SANTA, D.; PORCIANI, M.; POLI, A. COX-2 expression in canine and feline invasive mammary carcinomas: correlation with clinicopathological features and prognostic molecular markers. Breast Cancer Research and Treatment, v. 98, n. 1, p. 115-120, 2006.
MISDORP, W.; ELSE, R. W.; HELLMÉN, E. Histological classification of mammary tumors of the dog and the cat. Armed Forces Institute of Pathology, v. 7, n. 1, p.1-59, 1999.
MOULTON, J. E. Tumors of the mammary gland. In:____. Tumors in Domestic Animals. 3. ed. Los Angeles: University of California Press, 1990. Cap. 12, p. 518-552.
NIETO, A.; PENA, L.; PEREZ-ALENZA, M. D.; SANCHEZ, M. A.; FLORES, J. M.; CASTANO, M. Immunohistochemical detection of estrogen receptor alpha in canine mammary tumors: clinical and pathologic associations and prognostic significance. Veterinary Pathology, v. 37, p. 239-247, 2000.
O´REILLY, S. M.; RICHARDS, M. A: Clinical aspects of assessing cell proliferation. In: Assessment of Cell Proliferation in Clinical Practice, ed. Hall PA, Levison DA, and Wright NA, pp. 63-79. Springer, London, UK, 1992.
PEÑA, L. L.; NIETO, A. I.; PÉREZ-ALENZA, D.; CUESTA, P.; CASTAÑO, M. Immunohistochemical detection of Ki-67 and PCNA in canine mammary tumors: Relationship to clinical and pathologic variables. Journal of Veterinary Diagnostic Investigation, v. 10, n. 3, p. 237-346, 1998.
RUNGSIPIPAT, A.; TATEYAMA, S.; YAMAGUCHI, R.; UCHIDA, K.; MIYOSHI, N.; HAYASHI, T. Immunohistochemical analysis of c-yes and c-erbB-2 oncogene products and p53 tumor suppressor protein in canine mammary tumors. Journal of Veterinary Medical Science, v. 61, p. 27-32, 1999.
RUTTEMAN, G. R.; WITHROW, S. J.; MACEWEN, E. G. Tumors of the mammary gland. In: Small animal clinical oncology. 3 ed. Philadephia: Saunders., 2001, p. 455-467.
SAKAI, H.; NODA, A.; SHIRAI, N.; TIDAKA, T.; YANAI, T.; MASEGI, T. Proliferative Activity of Canine Mast Cell Tumors Evaluated by Bromodeoxyuridine Incorporation and Ki-67 Expression. Journal of Comparative Pathology, v. 127, n. 4, p. 233-238, 2002.
VELDHOEN, N.; WATTERSON, J.; BRASH, M.; MILNER, J. Identification of tumor-associated and germ line p53 mutations in canine mammary cancer. British Journal of Cancer, v. 81, p. 409-415, 1999.
YANG, W. Y.; LIU, C. H.; CHANG, C. J.; LEE, C. C.; CHANG, K. J.; LIN, C. T. Proliferative activity, apoptosis and expression of oestrogen receptor and Bcl-2 oncoprotein in canine mammary gland tumors. Journal of Comparative Pathology, v. 134, p. 70-79, 2006.
WU, H.; HAYASHI, T.; INOUE, M. Immunohistochemical expression of Mdm2 and p53 in canine cutaneous mast cell tumours. Journal of Veterinary Medicine – A Physiology, Pathology, Clinical Medicine, v. 53, n. 2, p. 65-68, 2006.
ZACCHETTI, A.; VAN GARDEREN, E.; TESKE, E.; NEDERBRAGT, H.; DIERENDONCK, J. H.; RUTTEMAN, G. R. Validation of the use of proliferation markers in canine neoplastic and non-neoplastic tissues: comparison of Ki-67 and proliferating cell nuclear antigen (PCNA) expression versus in vivo bromodeoxyuridine labeling by immunohistochemistry. Acta Pathologica, microbiologica et Immunologica, v. 111, p. 430-438, 2003.
ZUCCARI, D. A. P. C.; TERZIAN, A. C. B. ; PEREIRA, R. S.; PAVAM, M. V.; RUIZ, C. M.; UEIRO, F. A. R.; ANDRADE, J. C. A.; CORDEIRO, J. A. Avaliação imunohistoquímica do gene p53 nas neoplasias mamárias caninas. ARS Veterinária, v. 21, n. 3, p. 344-350, 2005.
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Copyright (c) 2012 Ana Carolina Trompieri-Silveira,Geórgia Modé Magalhães, Antonio Carlos Alessi
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