Isolation of Aspergillus spp. and Nocardia sp. in the inflammatory process of the mammary gland of bovine milk

Abstract

The mastitis is one of the main problems faced in dairy farming, it is characterized by inflammation of the mammary gland, with etiology of bacterial, fungal and viral origin among others. The present study aimed to report the occurrence of a case of bovine mastitis caused by Aspergillus spp. and Nocardia sp, in a co-infection in the Northwest region of Rio Grande do Sul. The milk sample was analyzed in the laboratory of Unijuí Veterinary Microbiology seeded in 5% sheep blood agar and McConkey agar. In the visual analysis of the fungus, the growth of a white, velvety-looking colony with approximately 2 cm in diameter and with its obverse of greenish coloration was observed, under microscopic examination, branched and septate hyphae, vesicle, phalidus and conidia, compatible with aspergillary characteristics, characterizing the isolation of Aspergillus spp. In the same sample, the presence of small colonies, with a smooth appearance, white-opaque staining and firmly attached to the agar, with the following characteristics, gram-positive, filamentous and branched colonies, with predominance of bacillary formats, were observed visually. identification of the genus Nocardia sp. From this case in this region, it is possible to emphasize the great importance of identifying the causative agent of mastitis, since mastitis caused by fungi, such as Aspergillus spp., Concomitantly with bacteria of the genus Nocardia sp., Although they are low several economic losses, due to the decrease in the quantity and quality of the milk produced.