Genotoxicity in Danio rerio exposed to increasing concentrations of soluble fractioning of burnt soybean oil biodiesel

Abstract

The objective of this research, to determine the presence of micronuclei in erythrocytes of juvenile fish zebrafish (Danio rerio) exposed to increasing soluble fraction concentrations of burnt soybean oil biodiesel (FSB). The fishes (N=15) were placed in polyethylene aquariums, with constant aeration, and submitted to acute toxicity test in a completely randomized design, with six treatments and two replicates. The treatments consisted of different FSB concentrations: solution I (control), solution II (0.0645 g L-1), solution III (0.129 g L-1), solution IV (0.645 g L-1), solution V (1.29 g L-1) and solution VI (6.45 g L-1). We collected a blood smear lamina for each fish, analyzing 2000 intact erythrocytes. There was significant difference (p<0.05) between groups I (control), II, III and IV and groups V and VI in the periods of 24 and 96 hours of exposition. With these results we may observe that the FSB presents a genotoxic effect in the erythrocytes of juvenile fish zebrafish.