Evaluation of fetal bovine serum as a source of enrichment in synthetic culture medium for the isolation of Mycobacterium bovis
DOI:
https://doi.org/10.31533/pubvet.v18n06e1612Keywords:
Culture, diagnosis, Middlebrook, OADC, bovine tuberculosisAbstract
A Middlebrook 7H10 medium added with 0.2% glucose and Fetal Bovine Serum (FBS) at concentrations of 5, 10, and 15% was compared with a Middlebrook 7H11 medium enriched with commercial OADC to evaluate the growth of Mycobacterium bovis, strain AN5, in the dilutions 10-1, 10-2, 10-3 and 10-4 performed from the McFarland scale 1. The results demonstrated micro and macroscopic growth of mycobacteria in all concentrations of SFB two weeks after inoculation, and in greater numbers when compared to Middlebrook 7H11 supplemented with commercial OADC. Statistical analysis found a significant reduction in the Middlebrook 7H11 group when compared to the 15% SFB concentration group in dilutions 10-2, 10-3, and 10-4. The results presented allowed us to conclude that FBS can be used as a source of enrichment for a Middlebrook 7H10 Medium in a thin layer, for isolation of Mycobacterium bovis, with the advantage of being more accessible and cheaper than the commercial medium.
References
Admassu, B., Kebede, E., & Shite, A. (2015). Review on bovine tuberculosis. Journal of Veterinary Advances, 5(3), 841. https://doi.org/20150315015831.
Ahuja, O. P., Bal, A., & Nath, K. (1967). Middlebrook-Dubos test in experimental ocular tuberculosis. American Journal of Ophthalmology, 63(6). https://doi.org/10.1016/0002-9394(67)93661-6.
Arnot, L. F., & Michel, A. (2020). Challenges for controlling bovine tuberculosis in South Africa. In Onderstepoort Journal of Veterinary Research (Vol. 87, Issue 1). https://doi.org/10.4102/ojvr.v87i1.1690.
Bertollo, D. M. B., & Montanha, J. O. M. (2022). Experiência exitosa no isolamento axênico de Leishmania infantum em meio de cultura enriquecido com sangue de equino. PUBVET, 16(4), 1–9. https://doi.org/10.31533/pubvet.v16n04a1090.1-9.
Bonnet, M., Lagier, J. C., Raoult, D., & Khelaifia, S. (2020). Bacterial culture through selective and non-selective conditions: The evolution of culture media in clinical microbiology. In New Microbes and New Infections (Vol. 34). https://doi.org/10.1016/j.nmni.2019.100622.
Borham, M., Oreiby, A., El-Gedawy, A., Hegazy, Y., Khalifa, H. O., Al-Gaabary, M., & Matsumoto, T. (2022). Review on bovine tuberculosis; na emerging disesase associated with multidrug-resistant Mycobacterium species. Pathogens, 11(715), 2–25. https://doi.org/10.3390/pathogens11070715.
Brittle, W., Marais, B. J., Hesseling, A. C., Schaaf, H. S., Kidd, M., Wasserman, E., & Botha, T. (2009). Improvement in mycobacterial yield and reduced time to detection in pediatric samples by use of a nutrient broth growth supplement. Journal of Clinical Microbiology, 47(5). https://doi.org/10.1128/JCM.02320-08.
Carneiro, P. A. M., Takatani, H., Pasquatti, T. N., Silva, C. B. D. G., Norby, B., Wilkins, M. J., Zumárraga, M. J., Araujo, F. R., & Kaneene, J. B. (2019). Epidemiological study of Mycobacterium bovis infection in buffalo and cattle in Amazonas, Brazil. Frontiers in Veterinary Science, 6, 434. https://doi.org/10.3389/fvets.2019.00434.
Corner, L. A. L., Gormley, E., & Pfeiffer, D. U. (2012). Primary isolation of Mycobacterium bovis from bovine tissues: Conditions for maximising the number of positive cultures. Veterinary Microbiology, 156(1–2). https://doi.org/10.1016/j.vetmic.2011.10.016.
Dib, C. C. (2023). Utilização do MALDI-TOF MS para identificação de micobactérias em amostras clínicas de animais. PUBVET, 17(7), e1414. https://doi.org/10.31533/pubvet.v17n7e1414.
Dib, C. C., Morais, Z. M., Souza, G. O., Amaku, M., Benites, N. R., & Pinheiro, S. R. (2006). Utilização de uma técnica rápida para diagnóstico de Mycobacterium bovis em amostras de leite experimentalmente inocualas. Arquivos Do Instituto Biológico, 73(2). https://doi.org/10.1590/1808-1657v73p1492006.
DIFCO. (2009). Manual of microbiological culture media. In Chemistry &
Dinno, A. (2015). Nonparametric pairwise multiple comparisons in independent groups using Dunn’s test. Stata Journal, 15(1). https://doi.org/10.1177/1536867x1501500117.
Fonseca, L. S., Silva, M. G., & Gontijo Filho, P. P. (1985). Avaliação da eficiência do meio 7H10 com soro fetal bovino no crescimento de microbactérias do complexo M tuberculosis. Revista de Microbiologia, 16(4), 272–274.
Gill, A. (2017). The importance of bacterial culture to food microbiology in the age of genomics. In Frontiers in Microbiology (Vol. 8, Issue MAY). https://doi.org/10.3389/fmicb.2017.00777.
Grange, J. M., & Yates, M. D. (1994). Zoonotic aspects of Mycobacterium bovis infection. Veterinary Microbiology, 40(1–2), 137–151.
Green, L. H. (2021). Culturing and preserving microorganisms. In Practical Handbook of Microbiology. https://doi.org/10.1201/9781003099277-4.
Hoffmann, E. R., Buss, B., Pereira, M. M., Reis, D., Teixeira, M. C., Moscon, L. A., Rondon, D. A., & Pereira, C. M. (2022). Tuberculose em bovinos no noroeste do Espírito Santo. PUBVET, 16(6), 1–5. https://doi.org/10.31533/pubvet.v16n06a1138.1-5.
Issa, M. A., Soares Filho, P. M., Fonseca Júnior, A. A., Hodon, M. A., Santos, L. C., Reis, J. K. P. dos, & Leite, R. C. (2017). Comparative study of Mycobacterium bovis primary isolation methods. Brazilian Journal of Microbiology, 48(1). https://doi.org/10.1016/j.bjm.2016.07.026.
Jochems, C. E. A., Van der Valk, J. B. F., Stafleu, F. R., & Baumans, V. (2002). The use of fetal bovine serum: Ethical or scientific problem? Alternatives to Laboratory Animals, 30(2). https://doi.org/10.1177/026119290203000208
Joloba, M. L., Johnson, J. L., Feng, P. J. I., Bozeman, L., Goldberg, S. V., Morgan, K., Gitta, P., Boom, H. W., Heilig, C. M., Mayanja-Kizza, H., & Eisenach, K. D. (2014). What is the most reliable solid culture medium for tuberculosis treatment trials? Tuberculosis, 94(3). https://doi.org/10.1016/j.tube.2014.03.002
Krajewska-Wedzina, M., Augustynowicz-Kopec, E., Weiner, M., & Szulowski, K. (2018). Treatment for active tuberculosis in giraffe (Giraffa camelopardalis) in a Zoo and potential consequences for public health-case report. Annals of Agricultural and Environmental Medicine, 25(4), 593–595. https://doi.org/10.26444/aaem/75685.
Lee, D. Y., Lee, S. Y., Yun, S. H., Jeong, J. W., Kim, J. H., Kim, H. W., Choi, J. S., Kim, G. D., Joo, S. T., Choi, I., & Hur, S. J. (2022). Review of the Current Research on Fetal Bovine Serum and the Development of Cultured Meat. In Food Science of Animal Resources (Vol. 42, Issue 5). https://doi.org/10.5851/kosfa.2022.e46
Marcondes, A. G., Shikama, M. D. L. M., Vasconcellos, S. A., Benites, N. R., Morais, Z. M. de, Roxo, E., Dias, R. A., Leão, S. L. P. C., & Pinheiro, S. R. (2006). Comparação entre a técnica de cultivo em camada delgada de ágar Middlebrook 7H11 e meio de Stonebrink para isolamento de Mycobacterium Bovis em amostras de campo. Brazilian Journal of Veterinary Research and Animal Science, 43(3). https://doi.org/10.11606/issn.1678-4456.bjvras.2006.26484
Mejia, G. I., Castrillon, L., Trujillo, H., & Robledo, J. A. (1999). Microcolony detection in 7H11 thin layer culture is an alternative for rapid diagnosis of Mycobacterium tuberculosis infection. International Journal of Tuberculosis and Lung Disease, 3(2).
Muniaraj, M., Lal, C. S., Kumar, S., Sinha, P. K., & Das, P. (2007). Milk of cow (Bos taurus), buffalo (Bubalus bubalis), and goat (Capra hircus): a better alternative than fetal bovine serum in media for primary isolation, in vitro cultivation, and maintenance of Leishmania donovani promastigotes. Journal of Clinical Microbiology, 45(4), 1353–1356.
Nascimento, A. J. S., & Nardi Júnior, G. (2022). Programa de controle e erradicação da brucelose e tuberculose animal. Tekhne e Logos, 13(2), 34–43.
Neves, E. D., Mezalira, T. S., Dias, E. H., Dourado, M. R., Paula, M. K., Gusman, C. R., Silva Caetano, I. C., Beltrami, J. M., & Otutumi, L. K. (2017). Lesões de tuberculose bovina em abatedouros-frigoríficos no Brasil: Bibliometria. Jornal Interdisciplinar de Biociências, 2(2), 22–27. https://doi.org/10.26694/jibi.v2i2.6171.
OMSA. (2022). Tuberculose bovina. OMSA.
Pérez-Lago, L., Navarro, Y., & García-de-Viedma, D. (2014). Current knowledge and pending challenges in zoonosis caused by Mycobacterium bovis: a review. Research in Veterinary Science, 97, S94–S100.
Pilgrim, C. R., McCahill, K. A., Rops, J. G., Dufour, J. M., Russell, K. A., & Koch, T. G. (2022). A Review of Fetal Bovine Serum in the Culture of Mesenchymal Stromal Cells and Potential Alternatives for Veterinary Medicine. In Frontiers in Veterinary Science (Vol. 9). https://doi.org/10.3389/fvets.2022.859025.
OIE (World Organisation for Animal Health). Chapter 3.4.6. (2018). Bovine tuberculosis. In: Terrestrial Manual, Disponível em: https://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/3.04.06_BOVINE_TB.pdf.
OIE (World Organisation for Animal Health). Chapter 3.1.13 (2022). Bovine tuberculosis. In: Terrestrial Manual, Disponível em: https://www.woah.org/fileadmin/Home/eng/Health_standards/tahm/3.01.13_Mammalian_tuberculosis.pdf.
Poester, F., Figueiredo, V. C. F., Lôbo, J. R., Gonçalves, V. S. P., Lage, A. P., Roxo, E., Mota, P. M. P. C., Müller, E. E., & Ferreira Neto, J. S. (2009). Estudos de prevalência da brucelose bovina no âmbito do Programa Nacional de Controle e Erradicação de Brucelose e Tuberculose. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 61, 1–5.
Rauch, C., Feifel, E., Amann, E.-M., Peter Spötl, H., Schennach, H., Pfaller, W., & Gstraunthaler, G. (2011). Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. ALTEX-Alternatives to Animal Experimentation, 28(4), 305–316.
Robledo, J. A., Mejía, G. I., Morcillo, N., Chacón, L., Camacho, M., Luna, J., Zurita, J., Bodon, A., Velasco, M., Palomino, J. C., Martin, A., & Portaels, F. (2006). Evaluation of a rapid culture method for tuberculosis diagnosis: A Latin American multi-center study. International Journal of Tuberculosis and Lung Disease, 10(6).
Rosário, T. R., Dib, C. C., Roxo, E., Pinheiro, S. R., Vasconcellos, S. A., & Benites, N. R. (2014). Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis. Brazilian Journal of Microbiology, 45(1). https://doi.org/10.1590/S1517-83822014005000038.
Sabagh, B. P., Souto, A. S. S., Reis, L. M., Silva, S. A., Pereira, D. C. R., Neves, M. C., Pinheiro, R. R., Duarte, R. S., Miyazaki, N. H. T., & Villas Bôas, M. H. S. (2012). Comparative study with two different enrichments in the culture media used in the disinfectant efficacy assay. Journal of Microbiological Methods, 88(2). https://doi.org/10.1016/j.mimet.2011.12.004.
Silva, P. E. A., Wiesel, F., Boffo, M. M. S., Von Groll, A., Mattos, I. G., Mejia, G., & Robledo, J. (2007). Microcolony detection in thin layer culture as an alternative method for rapid detection of Mycobacterium tuberculosis in clinical samples. Brazilian Journal of Microbiology, 38(3). https://doi.org/10.1590/s1517-83822007000300007.
Teppawar, R. N., Chaudhari, S. P., Moon, S. L., Shinde, S. V, Khan, W. A., & Patil, A. R. (2018). Zoonotic tuberculosis: A concern and strategies to combat. In Basic Biology and Applications of Actinobacteria. https://doi.org/10.5772/intechopen.76802.
Valente, L. C. M., Vale, S. M. L. R., & Braga, M. J. (2011). Determinantes do uso de medidas sanitárias de controle da brucelose e tuberculose bovinas. Revista de Economia e Sociologia Rural, 49(1), 215–231.
Zimpel, C. K., Patané, J. S. L., Guedes, A. C. P., Souza, R. F., Silva-Pereira, T. T., Camargo, N. C. S., de Souza Filho, A. F., Ikuta, C. Y., Neto, J. S. F., & Setubal, J. C. (2020). Global distribution and evolution of Mycobacterium bovis lineages. Frontiers in Microbiology, 11, 843. https://doi.org/10.3389/fmicb.2020.00843.
Universidad de las Naciones Unidas. (1998). Nuevas tecnologias para el diagnóstico y pruebas de susceptibilidad a drogas de M. tuberculosis para países en vias de desarollo. In: Programa de Biotecnologia para Latinoamérica y el Caribe – BIOLAC. Red Latinoamericana y del Caribe de tuberculosis – RELACTB. La Paz (Bolivia); 1998:18-21.
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Cristina Corsi Dib, Luara Lucena Cassiano
This work is licensed under a Creative Commons Attribution 4.0 International License.
Você tem o direito de:
Compartilhar — copiar e redistribuir o material em qualquer suporte ou formato
Adaptar — remixar, transformar, e criar a partir do material para qualquer fim, mesmo que comercial.
O licenciante não pode revogar estes direitos desde que você respeite os termos da licença. De acordo com os termos seguintes:
Atribuição
— Você deve dar o crédito apropriado, prover um link para a licença e indicar se mudanças foram feitas. Você deve fazê-lo em qualquer circunstância razoável, mas de nenhuma maneira que sugira que o licenciante apoia você ou o seu uso. Sem restrições adicionais
— Você não pode aplicar termos jurídicos ou medidas de caráter tecnológico que restrinjam legalmente outros de fazerem algo que a licença permita.
