Bisfenol A na indústria alimentícia. Análise comparativa entre carnes e produtos cárneos enlatados e não enlatados

Revisão

Autores

  • Alícia Ramos Buil Universidad Cardenal Herrera - Valencia, España
  • Sandra Fernández Pastor Universidad Cardenal Herrera - Valencia, España
  • Ana Guerrero Barrado Universidad Cardenal Herrera - Valencia, España

DOI:

https://doi.org/10.31533/pubvet.v18n09e1655

Palavras-chave:

Carne enlatada, carne fresca, embalagem de alimentos, Saúde Pública

Resumo

O Bisfenol A é uma substância química capaz de produzir efeitos nocivos à saúde humana e animal, dentre os quais se destacam alterações reprodutivas, neurológicas, metabólicas, digestivas e distúrbios do desenvolvimento. Apesar dessas contraindicações, seu uso é difundido mundialmente e muito procurado pela indústria devido às propriedades de resistência que oferece. No setor alimentício, é utilizado na fabricação de embalagens, especificamente em latas revestidas com resinas epóxi e em embalagens de policarbonato. Vários estudos científicos conseguiram confirmar que os produtos cárneos contêm quantidades significativas dela, tanto na forma enlatada como na forma fresca. Esta revisão bibliográfica pretende compreender o contexto jurídico em que se encontra o Bisfenol A, conhecer os níveis de ingestão diária toleráveis, analisar o seu impacto na saúde humana e animal, investigar as principais vias de exposição a esta substância e, em particular, aprofundar-se em possíveis transferências de Bisfenol A para produtos cárneos enlatados e frescos. Além disso, são exploradas alternativas ao seu uso, incluindo o uso de seus análogos. Esta revisão destaca a elevada presença de Bisfenol A em produtos cárneos e a necessidade de regulamentação legal mais rigorosa para preservar a saúde da população. Da mesma forma, conclui-se a urgência de maiores pesquisas científicas para encontrar alternativas mais seguras e sustentáveis ao seu uso.

Referências

ABPA. (2024). Associação brasileira de proteína animal. In Relatório Anual de Atividades.

ACSA. (2024). Agència Catalana de Seguretat Alimentària. Exposició alimentària al bisfenol A. In Exposició alimentària al bisfenol A.

AESAN. (2021). Agencia Española de Seguridad Alimentaria y Nutrición. Preguntas y respuestas sobre el bisfenol A.

Alabi, A., Caballero-Casero, N., & Rubio, S. (2014). Quick and simple sample treatment for multiresidue analysis of bisphenols, bisphenol diglycidyl ethers and their derivatives in canned food prior to liquid chromatography and fluorescence detection. Journal of Chromatography A, 1336, 23–33. https://doi.org/10.1016/j.chroma.2014.02.008.

Alvarado, J., Martínez, G., Navarrete, J., Botello, E., Calderón, M., & Jiménez, H. (2009). Fenomenología de la esterilización de alimentos líquidos enlatados. Revista Facultad de Ingeniería, 50. https://doi.org/10.17533/udea.redin.14934.

ANVISA. (2013). Manual de microbiologia clínica para o controle de infecção relacionada à assistência à saúde (D. e identificação de bactérias de importância médica, Ed.; Vol. 9). Ministério da Saúde.

Arnold, S. M., Clark, K. E., Staples, C. A., Klecka, G. M., Dimond, S. S., Caspers, N., & Hentges, S. G. (2013). Relevance of drinking water as a source of human exposure to bisphenol A. Journal of Exposure Science and Environmental Epidemiology, 23(2), 137–144. https://doi.org/10.1038/jes.2012.66.

Ben Maamar, M., Lesne, L., Desdoits-Lethimonier, C., Coiffec, I., Lassurguère, J., Lavoué, V., Deceuninck, Y., Antignac, J. P., Le Bizec, B., Perdu, E., Zalko, D., Pineau, C., Chevrier, C., Dejucq-Rainsford, N., Mazaud-Guittot, S., & Jégou, B. (2015). An investigation of the endocrine-disruptive effects of bisphenol A in human and rat fetal testes. PLoS ONE, 10(2). https://doi.org/10.1371/journal.pone.0117226.

Braun, J. M., Kalkbrenner, A. E., Calafat, A. M., Yolton, K., Ye, X., Dietrich, K. N., & Lanphear, B. P. (2011). Impact of early-life bisphenol A exposure on behavior and executive function in children. Pediatrics, 128(5), 873–882. https://doi.org/10.1542/peds.2011-1335.

Comisión Europea. (2011 a) Directiva 2011/8/UE de la Comisión, de 28 de enero de 2011 que modifica la Directiva 2002/72/CE por lo que se refiere a la restricción del uso de bisfenol A en biberones de plástico para lactantes. Diario Oficial de la Unión Europea, 29 de enero de 2011, no. 26, pp. 11-14. Disponible en: https://www.boe.es/doue/2011/026/L00011- 00014.pdf.

Comisión Europea. (2011 b) Reglamento (UE) 10/2011 de la Comisión, de 14 de enero de 2011 sobre materiales y objetos plásticos destinados a entrar en contacto con alimentos. Diario Oficial de la Unión Europea, 15 de enero de 2011, no. 12, pp. 1-89. Disponible en: https://www.boe.es/doue/2011/012/L00001-00089.pdf.

Comisión Europea. Reglamento (2016). Reglamento (UE) 2016/2235 de la Comisión, de 12 de diciembre de 2016 que modifica, por lo que respecta al bisfenol A, el anexo XVII del Reglamento (CE) n.º 1907/2006 del Parlamento Europeo y del Consejo, relativo al registro, la evaluación, la autorización y la restricción de las sustancias y mezclas químicas. Diario Oficial de la Unión Europea, 13 de diciembre de 2016, no. 337, pp. 3-5. Disponible en: https://www.boe.es/doue/2016/337/L00003-00005.pdf.

Comisión Europea. Reglamento (2018) Reglamento (UE) 2018/213 de la Comisión, de 12 de febrero de 2018 sobre el uso de bisfenol A en los barnices y revestimientos destinados a entrar en contacto con los alimentos y por el que se modifica el Reglamento (UE) n.º 10/2011 por lo que respecta al uso de dicha substancia en materiales plásticos en contacto con los alimentos. Diario Oficial de la Unión Europea, 14 de febrero de 2018, no. 41, pp. 6-12. Disponible en: https://www.boe.es/doue/2018/041/L00006-00012.pdf [Consulta: 24 marzo 2024].

Cooper, B. L., & Posnack, N. G. (2022). Characteristics of bisphenol cardiotoxicity: Impaired excitability, contractility, and relaxation. In Cardiovascular Toxicology (Vol. 22, Issue 3, pp. 273–280). https://doi.org/10.1007/s12012-022-09719-9.

Cunha, S. C., Inácio, T., Almada, M., Ferreira, R., & Fernandes, J. O. (2020). Gas chromatography–mass spectrometry analysis of nine bisphenols in canned meat products and human risk estimation. Food Research International, 135(109293). https://doi.org/10.1016/j.foodres.2020.109293.

Daronch, O. T., Lima, M. de M., Gil, I., Bittencourt, S. S., Ingenchki, V., & Maluf, E. M. C. P. (2020). Large-scale contamination by Bisphenol A: Are we aware of the risk and forms of exposure? Ciencia e Saude Coletiva, 25(11), 4339–4345. https://doi.org/10.1590/1413-812320202511.01852018.

EFSA. (2018). European food safety authority.

Engin, A. B., & Engin, A. (2021). The effect of environmental Bisphenol A exposure on breast cancer associated with obesity. Environmental Toxicology and Pharmacology, 81(103544). https://doi.org/10.1016/j.etap.2020.103544.

Errico, S., Bianco, M., Mita, L., Migliaccio, M., Rossi, S., Nicolucci, C., Menale, C., Portaccio, M., Gallo, P., Mita, D. G., & Diano, N. (2014). Migration of bisphenol A into canned tomatoes produced in Italy: Dependence on temperature and storage conditions. Food Chemistry, 160. https://doi.org/10.1016/j.foodchem.2014.03.085.

España. (2022). Ley 7/2022, de 8 de abril, de residuos y suelos contaminados para una economía circular”. Boletín Oficial del Estado, 10 de abril de 2022, no. 85, 1-136. Disponible en: https://www.boe.es/buscar/pdf/2022/BOE-A-2022-5809-consolidado.pdf.

FAOSTAT. (2022). Organización de las Naciones Unidas para la Alimentación y la Agricultura. Datos sobre alimentación y agricultura (Vol. 1, Issue 1).

Ferloni, A., Pereiro, N., Cruz, M., Aragone, S., Kandel Gambarte, P., Aliperti, V., Saulino, J. C., Cambiaso, O., Vázquez, M. L., Méndez, M. L., Moix, C. F., Vidal, F. A., Uicich, R., Otaño, L., Figar, S., Giménez, M. I., & Wahren, C. G. (2019). Fetal exposure to bisphenol A: Presence of bisphenol A in urine of pregnant women assisted in a hospital in the City of Buenos Aires. Year 2013. Revista de La Facultad de Ciencias Medicas (Cordoba, Argentina), 76(2), 86–91. https://doi.org/10.31053/1853.0605.v76.n2.22806.

Ferrer, E., Santoni, E., Vittori, S., Font, G., Mañes, J., & Sagratini, G. (2011). Simultaneous determination of bisphenol A, octylphenol, and nonylphenol by pressurised liquid extraction and liquid chromatography-tandem mass spectrometry in powdered milk and infant formulas. Food Chemistry, 126(1), 360–367. https://doi.org/10.1016/j.foodchem.2010.10.098.

Friques, A. G. F., Santos, F. D. N., Angeli, D. B., Silva, F. A. C., Dias, A. T., Aires, R., Leal, M. A. S., Nogueira, B. V., Amorim, F. G., Campagnaro, B. P., Pereira, T. M. C., Campos-Toimil, M., Meyrelles, S. S., & Vasquez, E. C. (2020). Bisphenol A contamination in infant rats: Molecular, structural, and physiological cardiovascular changes and the protective role of kefir. Journal of Nutritional Biochemistry, 75(108254). https://doi.org/10.1016/j.jnutbio.2019.108254.

García, J. A., Gallego, C., & Font, G. (2015). Toxicidad del Bisfenol A. Revista de Toxicologia, 32(2), 144–160.

Gatidou, G., Vassalou, E., & Thomaidis, N. S. (2010). Bioconcentration of selected endocrine disrupting compounds in the Mediterranean mussel, Mytilus galloprovincialis. Marine Pollution Bulletin, 60(11), 2111–2116. https://doi.org/10.1016/j.marpolbul.2010.07.003.

Gerassimidou, S., Geueke, B., Groh, K. J., Muncke, J., Hahladakis, J. N., Martin, O. V., & Iacovidou, E. (2023). Unpacking the complexity of the polyethylene food contact articles value chain: A chemicals perspective. In Journal of Hazardous Materials (Vol. 454, Issue 131422). https://doi.org/10.1016/j.jhazmat.2023.131422.

Gore, A. C., Chappell, V. A., Fenton, S. E., Flaws, J. A., Nadal, A., Prins, G. S., Toppari, J., & Zoeller, R. T. (2015). EDC-2: The Endocrine Society’s Second Scientific Statement on Endocrine-Disrupting Chemicals. In Endocrine Reviews (Vol. 36, Issue 6). https://doi.org/10.1210/er.2015-1010.

Guo, Y., Shi, W., Liu, Z., Sun, X., Wu, J., & Wu, Y. (2023). Bisphenol A alternatives continuously contribute to the endocrine disruption in cetaceans. Environment International, 171(107679). https://doi.org/10.1016/j.envint.2022.107679.

Harnett, K. G., Chin, A., & Schuh, S. M. (2021). BPA and BPA alternatives BPS, BPAF, and TMBPF, induce cytotoxicity and apoptosis in rat and human stem cells. Ecotoxicology and Environmental Safety, 216(112210). https://doi.org/10.1016/j.ecoenv.2021.112210.

Hassan, Z. K., Elobeid, M. A., Virk, P., Omer, S. A., Elamin, M., Daghestani, M. H., & Alolayan, E. M. (2012). Bisphenol a induces hepatotoxicity through oxidative stress in rat model. Oxidative Medicine and Cellular Longevity, 194829. https://doi.org/10.1155/2012/194829.

Hötzel, M. J., & Vandresen, B. (2022). Brazilians’ attitudes to meat consumption and production: Present and future challenges to the sustainability of the meat industry. Meat Science, 192(109893). https://doi.org/10.1016/j.meatsci.2022.108893.

Ismail, O. I., & El-Meligy, M. M. S. (2022). Curcumin ameliorated low dose-Bisphenol A induced gastric toxicity in adult albino rats. Scientific Reports, 12(10201). https://doi.org/10.1038/s41598-022-14158-1.

Instituto Galego de Promoción Económica. (2022). Informe Sector Conservero Estados Unidos. Disponible en: https://www.igape.gal/images/05-mais-igape/05-05-quensomos-internacional/antenas/eeuu/Informe_Sector_Conservero_en_EE.UU_Castellano.pdf

Jambor, T., Knizatova, N., & Lukac, N. (2021). Men´s reproductive alterations caused by Bisphenol A and Its analogues: A review. Physiological Research, 70(4), 643–656. https://doi.org/10.33549/physiolres.934742.

Jochmanová, I., Lazúrová, Z., Rudnay, M., Bačová, I., Mareková, M., & Lazúrová, I. (2015). Environmental estrogen bisphenol A and autoimmunity. Lupus, 24(4–5), 4–5. https://doi.org/10.1177/0961203314560205.

Kang, J. H., Asai, D., & Toita, R. (2023). Bisphenol A (BPA) and cardiovascular or cardiometabolic diseases. In Journal of Xenobiotics (Vol. 13, Issue 4, pp. 775–810). https://doi.org/10.3390/jox13040049.

Lambré, C., Barat Baviera, J. M., Bolognesi, C., Chesson, A., Cocconcelli, P. S., Crebelli, R., Gott, D. M., Grob, K., Mengelers, M., Mortensen, A., Rivière, G., Steffensen, I. L., Tlustos, C., Van Loveren, H., Vernis, L., Zorn, H., Dudler, V., Milana, M. R., Papaspyrides, C., … Lampi, E. (2022). Safety assessment of the process Cajas y Palets en una Economia Circular (CAPEC), used to recycle high-density polyethylene and polypropylene crates for use as food contact materials. EFSA Journal, 20(6). https://doi.org/10.2903/j.efsa.2022.7384.

Lee, K. T. (2010). Quality and safety aspects of meat products as affected by various physical manipulations of packaging materials. Meat Science, 86(1), 138–150. https://doi.org/http://dx.doi.org/10.1016/j.meatsci.2010.04.035

Li, P., Gan, Z., Li, Z., Wang, B., Sun, W., Su, S. J., & Ding, S. (2024). Occurrence and exposure evaluation of bisphenol A and its analogues in indoor and outdoor dust from China. Science of the Total Environment, 920(170833). https://doi.org/10.1016/j.scitotenv.2024.170833.

Liu, J., Wattar, N., Field, C. J., Dinu, I., Dewey, D., & Martin, J. W. (2018). Exposure and dietary sources of bisphenol A (BPA) and BPA-alternatives among mothers in the APrON cohort study. Environment International, 119, 319–326. https://doi.org/10.1016/j.envint.2018.07.001.

Liu, R., Cai, D., Li, X., Liu, B., Chen, J., Jiang, X., Li, H., Li, Z., Teerds, K., Sun, J., Bai, W., & Jin, Y. (2022). Effects of Bisphenol A on reproductive toxicity and gut microbiota dysbiosis in male rats. Ecotoxicology and Environmental Safety, 239(113623). https://doi.org/10.1016/j.ecoenv.2022.113623.

Makowska, K., Staniszewska, M., Bodziach, K., Calka, J., & Gonkowski, S. (2022). Concentrations of bisphenol a (BPA) in fresh pork loin meat under standard stock-farming conditions and after oral exposure – A preliminary study. Chemosphere, 295(133816). https://doi.org/10.1016/j.chemosphere.2022.133816.

Mao, L., Fang, S., Zhao, M., Liu, W., & Jin, H. (2021). Effects of Bisphenol A and Bisphenol S Exposure at low doses on the metabolome of adolescent male Sprague-Dawley rats. Chemical Research in Toxicology, 34(6), 1578–1587. https://doi.org/10.1021/acs.chemrestox.1c00018,

Martínez-Arriagada, N., & Meriño-Castro, B. (2019). Concentración de Bisfenol A como disruptor endocrino en enlatados chilenos de la región metropolitana. Universidad Finis Terrae.

Michałowicz, J. (2014). Bisphenol A - Sources, toxicity and biotransformation. In Environmental Toxicology and Pharmacology (Vol. 37, Issue 2, pp. 738–758). https://doi.org/10.1016/j.etap.2014.02.003.

Mikołajewska, K., Stragierowicz, J., & Gromadzińska, J. (2015). Bisphenol A – Application, sources of exposure and potential risks in infants, children and pregnant women. In International Journal of Occupational Medicine and Environmental Health (Vol. 28, Issue 2, pp. 209–241). https://doi.org/10.13075/ijomeh.1896.00343.

Ministerio de Agricultura, Pesca y Alimentación. (2022). Informe del Consumo Alimentario en España 2022. Disponible en: https://www.mapa.gob.es/es/alimentacion/temas/consumo- tendencias/informe-consumo-2022-baja-res_tcm30-655390.pdf.

Mikołajewska, K., Stragierowicz, J., & Gromadzińska, J. (2015). Bisphenol A- Application, sources of exposure and potential risks in infants, children and pregnant women. International Journal of Occupational Medicine and Environmental Health, 28(2), 209–241. DOI: https://doi.org/10.13075/ijomeh.1896.00343.

Mordor Intelligence. (2024). Análisis de participación y tamaño del mercado de carne enlatada tendencias de crecimiento y pronósticos (2024-2029). Disponible en: https://www.mordorintelligence.com/es/industry-reports/global-canned-meat-market.

Muñoz, M. D. (2000). Sistema de tratamiento de aguas residuales de matadero: Para una población menor 2000 habitantes. Facultad de Ciencias Agropecuarias, 3(1), 87–98.

Nunes, C. L., Pflanzer, S. B., Souza, J. H. R., & Chizzotti, M. L. (2024). Beef production and carcass evaluation in Brazil. Animal Frontiers: The Review Magazine of Animal Agriculture, 14(2), 15–20. https://doi.org/10.1093/af/vfad074.

OCU. Organización de Consumidores y Usuarios. (2024). Bisfenol A en alimentos: un riesgo real para todos. Disponible en: https://www.ocu.org/alimentacion/seguridad-alimentaria/noticias/riesgo-bisfenol-a.

Pérez-Lobato, R., Mustieles, V., Calvente, I., Jiménez-Díaz, I., Ramos, R., Caballero-Casero, N., López-Jiménez, F. J., Rubio, S., Olea, N., & Fernandez, M. F. (2016). Exposure to bisphenol A and behavior in school-age children. NeuroToxicology, 53, 12–19. https://doi.org/10.1016/j.neuro.2015.12.001.

Pivonello, C., Muscogiuri, G., Nardone, A., Garifalos, F., Provvisiero, D. P., Verde, N., De Angelis, C., Conforti, A., Piscopo, M., Auriemma, R. S., Colao, A., & Pivonello, R. (2020). Bisphenol A: An emerging threat to female fertility. In Reproductive Biology and Endocrinology (Vol. 18, Issue 1, p. 22). https://doi.org/10.1186/s12958-019-0558-8.

Prabhu, N. B., Adiga, D., Kabekkodu, S. P., Bhat, S. K., Satyamoorthy, K., & Rai, P. S. (2022). Bisphenol A exposure modulates reproductive and endocrine system, mitochondrial function and cellular senescence in female adult rats: A hallmarks of polycystic ovarian syndrome phenotype. Environmental Toxicology and Pharmacology, 96. https://doi.org/10.1016/j.etap.2022.104010

Ramírez, V., Merkel, S., Tietz, T., & Rivas, A. (2023). Risk assessment of food contact materials. EFSA Journal, 21(S1), 1–10. https://doi.org/10.2903/j.efsa.2023.e211015.

Richter, C. A., Birnbaum, L. S., Farabollini, F., Newbold, R. R., Rubin, B. S., Talsness, C. E., Vandenbergh, J. G., Walser-Kuntz, D. R., & vom Saal, F. S. (2007). In vivo effects of bisphenol A in laboratory rodent studies. In Reproductive Toxicology (Vol. 24, Issue 2, pp. 199–224). https://doi.org/10.1016/j.reprotox.2007.06.004.

Russo, G., Barbato, F., Mitra, D. G., & Grumetto, L. (2019). Occurrence of Bisfenol A and its analogues in some foodstuff marketed in Europe. Food and Chemical Toxicology, 131(110575). https://doi.org/10.1016/j.fct.2019.110575.

Sasso, A. F., Pirow, R., Andra, S. S., Church, R., Nachman, R. M., Linke, S., Kapraun, D. F., Schurman, S. H., Arora, M., Thayer, K. A., Bucher, J. R., & Birnbaum, L. S. (2020). Pharmacokinetics of bisphenol A in humans following dermal administration. Environment International, 144(106031). https://doi.org/10.1016/j.envint.2020.106031.

Shi, R., Liu, Z., & Liu, T. (2021). The antagonistic effect of bisphenol A and nonylphenol on liver and kidney injury in rats. Immunopharmacology and Immunotoxicology, 43(5), 527–535. https://doi.org/10.1080/08923973.2021.1950179.

Siddique, A. B., Harrison, S. M., Monahan, F. J., Cummins, E., & Brunton, N. P. (2021). Bisphenol a and metabolites in meat and meat products: Occurrence, toxicity, and recent development in analytical methods. In Foods (Vol. 10, Issue 4, p. 714). https://doi.org/10.3390/foods10040714.

Siracusa, J. S., Yin, L., Measel, E., Liang, S., & Yu, X. (2018). Effects of bisphenol A and its analogs on reproductive health: A mini review. In Reproductive Toxicology (Vol. 79, pp. 96–123). https://doi.org/10.1016/j.reprotox.2018.06.005.

Srivastava, S., & Dhagga, N. (2019). Dose exposure of Bisphenol- A on female Wistar rats fertility. Hormone Molecular Biology and Clinical Investigation, 38(2). https://doi.org/10.1515/hmbci-2018-0061.

Stojanović, B., Radović, L., Natić, D., Dodevska, M., Vraštanović-Pavičević, G., Balaban, M., Lević, S., Petrović, T., & Antić, V. (2019). Influence of a storage conditions on migration of bisphenol A from epoxy-phenolic coating to canned meat products. Journal of the Serbian Chemical Society, 84(4), 377–389. https://doi.org/10.2298/JSC181015100S.

Wang, X., Nag, R., Brunton, N. P., Siddique, M. A. B., Harrison, S. M., Monahan, F. J., & Cummins, E. (2022). Human health risk assessment of bisphenol A (BPA) through meat products. In Environmental Research (Vol. 213, Issue 113734). https://doi.org/10.1016/j.envres.2022.113734.

Wang, X., Nag, R., Brunton, N. P., Siddique, M. A. B., Harrison, S. M., Monahan, F. J., & Cummins, E. (2023a). A probabilistic approach to model bisphenol A (BPA) migration from packaging to meat products. Science of the Total Environment, 854(158815). https://doi.org/10.1016/j.scitotenv.2022.158815.

Wang, X., Nag, R., Brunton, N. P., Siddique, M. A. B., Harrison, S. M., Monahan, F. J., & Cummins, E. (2023b). Risk assessment of bisphenol A (BPA) in Irish meat and meat products. Science of the Total Environment, 881(163496). https://doi.org/10.1016/j.scitotenv.2023.163496.

Wu, W., Li, M., Liu, A., Wu, C., Li, D., Deng, Q., Zhang, B., Du, J., Gao, X., & Hong, Y. (2020). Bisphenol A and the risk of obesity a systematic review with meta-analysis of the epidemiological evidence. In Dose-Response (Vol. 18, Issue 2). https://doi.org/10.1177/1559325820916949.

Zulkifli, S., Rahman, A. A., Kadir, S. H. S. A., & Nor, N. S. M. (2021). Bisphenol A and its effects on the systemic organs of children. In European Journal of Pediatrics (Vol. 180, Issue 10, pp. 3111–3127). https://doi.org/10.1007/s00431-021-04085-0.

Evolución de la Ingesta Diaria Tolerable (IDT) de Bisfenol A (BPA) durante el año 2015 y 2023 según evaluación de la EFSA.  Fuente: EFSA (2018) e Lambré et al. (2022).

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27-08-2024

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Ciência e tecnologia de alimentos

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Bisfenol A na indústria alimentícia. Análise comparativa entre carnes e produtos cárneos enlatados e não enlatados: Revisão. (2024). Pubvet, 18(09), e1655. https://doi.org/10.31533/pubvet.v18n09e1655